OBJECTIVE:
To contribute my strong technical background and diversified technical skills in areas of tissue engineering and biomaterials to your company in a Research Scientist’s capacity.
EDUCATION:
Biomedical Engineering, Doctor of Philosophy
Dec 2008 - Present
Virginia Commonwealth University, Richmond, VA
Doctoral Candidate (Nov 2010)
Biomedical Engineering, Master of Science
Aug 2006 - Dec 2008
Virginia Commonwealth University, Richmond, VA
GPA 3.7/4.0
Biomedical Engineering, Bachelor of Engineering
Aug 2002 - May 2006
Avinashilingam Deemed University, Tamil Nadu, India
GPA 8.9/10.0
TRAINING AND WORKSHOPS:
Six Sigma (Blue Belt) Training Feb 2012
Dominion, Richmond, VA
Learned core concepts and key skills for analyzing processes (CT tree, SIPOC, Detailed Process Map) with templates, examples and exercises.
Analog Devices Workshop June 2005
Indian Institute of Technology, Madras, India
Developed assembly code to implement various Digital Signal Processing applications (including image processing, speech coding and echo cancellation) using Blackfin ADSP-BF533 processor.
Hospital Summer Training May 2004-June 2004
Sir Ganga Ram Hospital, New Delhi, India
Training included viewing of clinical procedures and surgeries. The implementation of biomedical devices and equipment in major departments of the hospital was observed and documented.
RESEARCH EXPERIENCE AND TECHNICAL SKILLS:
• Cell Isolation: Bone marrow derived macrophages and mast cells.
• Cell Culture: Static and Dynamic (NASA’s rotating wall vessel 3D bioreactor).
• Polymer Scaffold Fabrication: Natural (Collagen & Silk isolation, processing and crosslinking and Elastin), Synthetic (Polycaprolactone and polydioxanone).
• Scaffold Testing: Uniaxial mechanical testing, pore size, permeability, fiber diameter, surface area to volume ratio measurement.
• Decellularization of Tissues: Porcine and Bovine Corium.
• Immunological and biological Assays: Enzyme Linked Immunosorbent Assay (ELISA), Western Blotting, immunohistology, RT-PCR.
• Wound healing & Angiogenesis Assays: Aortic ring, 3D bead sprouting assay, Scratch test.
• Microscopy: Brightfield/Fluorescent Microscope, Scanning Electron Microscope (SEM)
• Statistical Analysis: JMP® 8, SigmaPlot 11.
• Software Programs: C/C++, MATLAB®, ImageJ 1.44, UTHSCSA ImageTool 3.0, Adobe Dreamweaver, Macromedia Flash 8, Photoshop, Illustrator, MS Word, Excel, Powerpoint.
PEER REVIEWED JOURNAL ARTICLES:
1. Garg K, Bowlin GL., (2011), Electrospinning Jets and Nanofibrous Structures, Biomicrofluidics, 5(1): 013403.
2. Garg K, Ryan JJ, Bowlin GL., (2011), Modulation of Mast Cell Adhesion, Proliferation and Cytokine Secretion on Electrospun Bioresorbable Vascular Grafts, Journal of Biomedical Materials Research: Part A. 97(4):405-13.
3. Sell SA, Wolfe PS, Garg K, McCool JM, Rodriguez IA, Bowlin GL., (2010), The Use of Natural Polymers in Tissue Engineering: A Focus on Electrospun Extracellular Matrix Analogues. Polymers 2(4), 522-553.
4. Garg K, Bowlin GL., (2010), Creation of Bioresorbable Vascular Prosthetics Using Electrospinning, Polymer Preprints, 51(2), 90.
5. Garg K, Sell SA, Madurantakam P, Bowlin GL., (2009), Angiogenic Potential of Human Macrophages on Electrospun Bioresorbable Vascular Grafts, Biomedical Materials. 4(3), 031001.
6. Wolfe PS, Madurantakam P, Garg K, Sell SA, Beckman MJ, Bowlin GL., (2009), Evaluation Of Thrombogenic Potential of Electrospun Bioresorbable Vascular Graft Materials: Acute Monocyte Tissue Factor Expression, Journal of Biomedical Materials Research: Part A, 92(4):1321-8.
7. Sell SA, McClure MJ, Garg K, Wolfe PS., (2009), Electrospinning of Collagen / Biopolymers for Regenerative Medicine and Cardiovascular Tissue Engineering, Advanced Drug Delivery Reviews, 61(12), 1007-19.
8. Sell SA, Francis MP, Garg K, McClure MJ, Simpson DG, Bowlin GL., (2008), “Cross-Linking Methods of Electrospun Fibrinogen Scaffolds for Tissue Engineering Applications”, Biomedical Materials. 3(4):045001.
BOOK SECTION:
1. McClure MJ, Garg K, Rodriguez IA, McCool JM, Bowlin GL., (2011), ECM analogues obtained by electrospun nanofibers, In Electrospinning for Advanced Biomedical Applications and Therapies, iSmithers Rapra Publishing, Submitted.
2. Garg K, Sell SA and Bowlin GL., (2009), Electrospinning and Its Influence on the Structure of Polymeric Nanofibers, In Handbook of Textile Fibre Structure. Woodhead Publishing in Textiles: Number 88, Eichhorn, S.; Hearle, J.W.S.; Jaffe, M. and T. Kikutani, Eds. Woodhead Publishing Limited, Cambridge, UK, 958-981.
CONFERENCE PRESENTATIONS AND ABSTRACTS:
1. Garg K, Pullen NA, Ryan JJ, Bowlin GL., (2011) Macrophage polarization in response to varying fiber dimensions of electrospun bioresorbable vascular grafts, 9th World Biomaterials Congress (WBC), Chengdu, China, abstract submitted.
2. Garg K, Bowlin GL., (2010) Nonwoven Bioresorbable Vascular Grafts, 4th International Conference on Technical Textiles and Nonwovens, New Delhi, India.
3. Garg K, Ryan JJ, Bowlin GL., (2010), Modulation of Mast Cell Adhesion, Proliferation and Cytokine Secretion on Electrospun Bioresorbable Vascular Grafts, Poster presented at International Society for Applied Cardiovascular Biology’s (ISACB) 12th Biennial Meeting, Boston, MA...
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